Efficacy of Four Disinfectants Against Hepatitis B Virus

Background and Aim: Hepatitis B virus (HBV) is an important infectious agent in denti-stry necessitating the use of disinfectants to prevent its transmission. This study compared the efficacy of 2/100 sodium hypochlorite disinfectants prepared from 5% solution manufactured by four different manufacturers namely Ashimashi, Paknaz, Vitex and Active for disinfection of surfaces infected with HBV. Materials and Methods: In this in vitro experimental study, sera of 10 hepatitis B pa-tients were poured into microtubes and 2/100 sodium hypochlorite solutions prepared from 5% Ashimashi, Paknaz, Vitex and Active disinfectants were added to them in 1/1 ratio. Polymerase chain reaction (PCR) was performed using virus diagnostic kits to detect the viral genome. Real-time PCR was performed before and after incubation with the disinfectants to assess the viral load in the serum. The reduction in the viral load of HBV was statistically analyzed by Kruskal-Wallis and Mann-Whitney U tests. Results: No significant antiviral efficacy was noted following the application of Ashima-shi 2/100 sodium hypochlorite disinfectant. Paknaz showed the highest efficacy against HBV. Vitex and Active ranked next with significant differences (P<0.0001) Conclusion: Under the study limitations, Paknaz 2/100 sodium hypochlorite solution was the most effective while Ashimashi 2/100 sodium hypochlorite disinfectant did not show adequate efficacy against HBV.